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tet responsive promoter p tight  (Addgene inc)


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    Structured Review

    Addgene inc tet responsive promoter p tight
    Tet Responsive Promoter P Tight, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tet+responsive+promoter+p+tight/tet+operator+(Plasmid+%238901)/pmc10901740-295-88-108
    Average 92 stars, based on 17 article reviews
    tet responsive promoter p tight - by Bioz Stars, 2026-10
    92/100 stars

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    Related Articles

    Cloning:

    Article Title: A model of human neural networks reveals NPTX2 pathology in ALS and FTLD
    Article Snippet: .. Using NEBuilder HiFi DNA Assembly Cloning Kit (NEB E5520; HiFi kit), human wild-type, full-length TDP-43 with a C-terminal HA tag from a published pcDNA5 plasmid containing the human TDP-43 cDNA sequence , was inserted into our all-in-one monocistronic TetON (mTRE; build based on Markusic et. al. ), which was previously inserted into a pLVX lentiviral transfer vector (Clontech 632164), while deleting CMV-PGK-Puro, generating mTRE-TDP-43–HA lentiviral transfer vector. mTRE cassette was build using both NEBuilder and Q5 site directed mutagenesis (NEB E0554S; Q5 kit) kits and consists of the Tet-responsive promoter P tight , consisting of seven tet operator sequences followed by the minimal CMV promoter (sequence source pCW57.1; Addgene #41393), driving the inducible expression of the downstream TDP-43–HA, followed by downstream IRES2 sequence (sequence source Addgene #60857), which is immediately followed by T7 tag fused to SV40 NLS, which was fused to rtTA-Advanced (sequence source Addgene #41393), which made the rtTA predominantly nuclear, making it readily available for the system, while the T7 tag made rtTA a useful, independent marker of the transgenic cells. ..

    Plasmid Preparation:

    Article Title: A model of human neural networks reveals NPTX2 pathology in ALS and FTLD
    Article Snippet: .. Using NEBuilder HiFi DNA Assembly Cloning Kit (NEB E5520; HiFi kit), human wild-type, full-length TDP-43 with a C-terminal HA tag from a published pcDNA5 plasmid containing the human TDP-43 cDNA sequence , was inserted into our all-in-one monocistronic TetON (mTRE; build based on Markusic et. al. ), which was previously inserted into a pLVX lentiviral transfer vector (Clontech 632164), while deleting CMV-PGK-Puro, generating mTRE-TDP-43–HA lentiviral transfer vector. mTRE cassette was build using both NEBuilder and Q5 site directed mutagenesis (NEB E0554S; Q5 kit) kits and consists of the Tet-responsive promoter P tight , consisting of seven tet operator sequences followed by the minimal CMV promoter (sequence source pCW57.1; Addgene #41393), driving the inducible expression of the downstream TDP-43–HA, followed by downstream IRES2 sequence (sequence source Addgene #60857), which is immediately followed by T7 tag fused to SV40 NLS, which was fused to rtTA-Advanced (sequence source Addgene #41393), which made the rtTA predominantly nuclear, making it readily available for the system, while the T7 tag made rtTA a useful, independent marker of the transgenic cells. ..

    Sequencing:

    Article Title: A model of human neural networks reveals NPTX2 pathology in ALS and FTLD
    Article Snippet: .. Using NEBuilder HiFi DNA Assembly Cloning Kit (NEB E5520; HiFi kit), human wild-type, full-length TDP-43 with a C-terminal HA tag from a published pcDNA5 plasmid containing the human TDP-43 cDNA sequence , was inserted into our all-in-one monocistronic TetON (mTRE; build based on Markusic et. al. ), which was previously inserted into a pLVX lentiviral transfer vector (Clontech 632164), while deleting CMV-PGK-Puro, generating mTRE-TDP-43–HA lentiviral transfer vector. mTRE cassette was build using both NEBuilder and Q5 site directed mutagenesis (NEB E0554S; Q5 kit) kits and consists of the Tet-responsive promoter P tight , consisting of seven tet operator sequences followed by the minimal CMV promoter (sequence source pCW57.1; Addgene #41393), driving the inducible expression of the downstream TDP-43–HA, followed by downstream IRES2 sequence (sequence source Addgene #60857), which is immediately followed by T7 tag fused to SV40 NLS, which was fused to rtTA-Advanced (sequence source Addgene #41393), which made the rtTA predominantly nuclear, making it readily available for the system, while the T7 tag made rtTA a useful, independent marker of the transgenic cells. ..

    Mutagenesis:

    Article Title: A model of human neural networks reveals NPTX2 pathology in ALS and FTLD
    Article Snippet: .. Using NEBuilder HiFi DNA Assembly Cloning Kit (NEB E5520; HiFi kit), human wild-type, full-length TDP-43 with a C-terminal HA tag from a published pcDNA5 plasmid containing the human TDP-43 cDNA sequence , was inserted into our all-in-one monocistronic TetON (mTRE; build based on Markusic et. al. ), which was previously inserted into a pLVX lentiviral transfer vector (Clontech 632164), while deleting CMV-PGK-Puro, generating mTRE-TDP-43–HA lentiviral transfer vector. mTRE cassette was build using both NEBuilder and Q5 site directed mutagenesis (NEB E0554S; Q5 kit) kits and consists of the Tet-responsive promoter P tight , consisting of seven tet operator sequences followed by the minimal CMV promoter (sequence source pCW57.1; Addgene #41393), driving the inducible expression of the downstream TDP-43–HA, followed by downstream IRES2 sequence (sequence source Addgene #60857), which is immediately followed by T7 tag fused to SV40 NLS, which was fused to rtTA-Advanced (sequence source Addgene #41393), which made the rtTA predominantly nuclear, making it readily available for the system, while the T7 tag made rtTA a useful, independent marker of the transgenic cells. ..

    Expressing:

    Article Title: A model of human neural networks reveals NPTX2 pathology in ALS and FTLD
    Article Snippet: .. Using NEBuilder HiFi DNA Assembly Cloning Kit (NEB E5520; HiFi kit), human wild-type, full-length TDP-43 with a C-terminal HA tag from a published pcDNA5 plasmid containing the human TDP-43 cDNA sequence , was inserted into our all-in-one monocistronic TetON (mTRE; build based on Markusic et. al. ), which was previously inserted into a pLVX lentiviral transfer vector (Clontech 632164), while deleting CMV-PGK-Puro, generating mTRE-TDP-43–HA lentiviral transfer vector. mTRE cassette was build using both NEBuilder and Q5 site directed mutagenesis (NEB E0554S; Q5 kit) kits and consists of the Tet-responsive promoter P tight , consisting of seven tet operator sequences followed by the minimal CMV promoter (sequence source pCW57.1; Addgene #41393), driving the inducible expression of the downstream TDP-43–HA, followed by downstream IRES2 sequence (sequence source Addgene #60857), which is immediately followed by T7 tag fused to SV40 NLS, which was fused to rtTA-Advanced (sequence source Addgene #41393), which made the rtTA predominantly nuclear, making it readily available for the system, while the T7 tag made rtTA a useful, independent marker of the transgenic cells. ..

    Marker:

    Article Title: A model of human neural networks reveals NPTX2 pathology in ALS and FTLD
    Article Snippet: .. Using NEBuilder HiFi DNA Assembly Cloning Kit (NEB E5520; HiFi kit), human wild-type, full-length TDP-43 with a C-terminal HA tag from a published pcDNA5 plasmid containing the human TDP-43 cDNA sequence , was inserted into our all-in-one monocistronic TetON (mTRE; build based on Markusic et. al. ), which was previously inserted into a pLVX lentiviral transfer vector (Clontech 632164), while deleting CMV-PGK-Puro, generating mTRE-TDP-43–HA lentiviral transfer vector. mTRE cassette was build using both NEBuilder and Q5 site directed mutagenesis (NEB E0554S; Q5 kit) kits and consists of the Tet-responsive promoter P tight , consisting of seven tet operator sequences followed by the minimal CMV promoter (sequence source pCW57.1; Addgene #41393), driving the inducible expression of the downstream TDP-43–HA, followed by downstream IRES2 sequence (sequence source Addgene #60857), which is immediately followed by T7 tag fused to SV40 NLS, which was fused to rtTA-Advanced (sequence source Addgene #41393), which made the rtTA predominantly nuclear, making it readily available for the system, while the T7 tag made rtTA a useful, independent marker of the transgenic cells. ..

    Transgenic Assay:

    Article Title: A model of human neural networks reveals NPTX2 pathology in ALS and FTLD
    Article Snippet: .. Using NEBuilder HiFi DNA Assembly Cloning Kit (NEB E5520; HiFi kit), human wild-type, full-length TDP-43 with a C-terminal HA tag from a published pcDNA5 plasmid containing the human TDP-43 cDNA sequence , was inserted into our all-in-one monocistronic TetON (mTRE; build based on Markusic et. al. ), which was previously inserted into a pLVX lentiviral transfer vector (Clontech 632164), while deleting CMV-PGK-Puro, generating mTRE-TDP-43–HA lentiviral transfer vector. mTRE cassette was build using both NEBuilder and Q5 site directed mutagenesis (NEB E0554S; Q5 kit) kits and consists of the Tet-responsive promoter P tight , consisting of seven tet operator sequences followed by the minimal CMV promoter (sequence source pCW57.1; Addgene #41393), driving the inducible expression of the downstream TDP-43–HA, followed by downstream IRES2 sequence (sequence source Addgene #60857), which is immediately followed by T7 tag fused to SV40 NLS, which was fused to rtTA-Advanced (sequence source Addgene #41393), which made the rtTA predominantly nuclear, making it readily available for the system, while the T7 tag made rtTA a useful, independent marker of the transgenic cells. ..



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